Journal: Viruses

Article Title: Efficacy of Plant-Made Human Recombinant ACE2 against COVID-19 in a Golden Syrian Hamster Model

doi: 10.3390/v15040964

Figure Lengend Snippet: Antiviral activity of hrACE2 and hrACE2-Fd against SARS-CoV-2 in Vero E6 cells. ( A ) Antiviral efficacy of hrACE2 and hrACE2-Fd against SARS-CoV-2 was quantified using real-time RT-PCR. ( B ) Antiviral efficacy of hrACE2 and hrACE2-Fd against SARS-CoV-2 was quantified using plaque assay. hrACE2, human recombinant angiotensin-converting enzyme 2; Fd, foldon; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.

Article Snippet: The African green monkey kidney Vero E6 cell line (VERO 76) was purchased from the Korean Cell Line Bank (KCLB no. 21587) and maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Activity Assay, Quantitative RT-PCR, Plaque Assay, Recombinant

Journal: Viruses

Article Title: Efficacy of Plant-Made Human Recombinant ACE2 against COVID-19 in a Golden Syrian Hamster Model

doi: 10.3390/v15040964

Figure Lengend Snippet: Viral titer in nasal turbinate tissue. Hamsters were intranasally inoculated with 100 µL of 10 5 TCID 50 /mL SARS-CoV-2, and 2.5 mg/kg of hrACE2 or 2.5 mg/kg of hrACE2-Fd recombinant protein was intraperitoneally injected for 5 days (1–5 dpi). Viral titer in nasal turbinate tissue was measured by TCID 50 using Vero E6 cells at 3, 6, and 9 dpi. ACE2, angiotensin-converting enzyme 2; Fd, foldon; TCID 50 , 50% tissue culture infectious dose.

Article Snippet: The African green monkey kidney Vero E6 cell line (VERO 76) was purchased from the Korean Cell Line Bank (KCLB no. 21587) and maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Recombinant, Injection

Journal: Viruses

Article Title: Efficacy of Plant-Made Human Recombinant ACE2 against COVID-19 in a Golden Syrian Hamster Model

doi: 10.3390/v15040964

Figure Lengend Snippet: Viral titer in the lung tissue. Hamsters were intranasally inoculated with 100 µL of 10 5 TCID 50 /mL SARS-CoV-2, and 2.5 mg/kg of hrACE2 or 2.5 mg/kg of hrACE2-Fd recombinant protein was intraperitoneally injected for 5 days (1–5 dpi). Viral titer in lung tissue was measured by TCID 50 using Vero E6 cells at 3, 6, and 9 dpi. ACE2, angiotensin-converting enzyme 2; Fd, foldon; TCID 50 , 50% tissue culture infectious dose.

Article Snippet: The African green monkey kidney Vero E6 cell line (VERO 76) was purchased from the Korean Cell Line Bank (KCLB no. 21587) and maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Recombinant, Injection